Biotech

London - North West

£50k – £60k

Description

A venture-backed biotechnology company has an exciting opportunity for a Senior Protein Engineer to join its team. As Senior Protein Engineer you will be immobilising enzymes for cell-free biocatalysis and looking to improve catalyst performance through protein design, DNA design, cloning, protein expression, enzymatic assays and general biochemical methods.

Your day-to-day may involve enzyme design, cloning, protein expression, enzyme characterisation using assays and general biochemical methods, project management and leading others.

Senior Protein Scientist responsibilities

• Perform and troubleshoot experiments independently, supporting others to do the same.
• Design DNA constructs for cloning and expression of recombinant enzymes.
• Lead protein expression across a range of scales (1mL microwell - 500mL shake flasks).
• Characterise enzymes and proteins using established biochemical and/or enzymatic assays, participating in the assay development process where needed.
• Generate quantitative scientific data that is accurate and precise.
• Ensure the timely reporting of all SOPs internal/external reports at a high standard.

Senior Protein Scientist requirements

• Degree level qualification (ideally PhD) in a biological discipline or closely related.
• Experience in microbial enzyme expression and characterisation in industry or as a Postdoc.
• Experience with traditional and/or scarless DNA cloning methods.
• Experience with recombinant protein expression in E. coli and troubleshooting difficult expressions (yield, toxicity, solubility issues).
• Comfortable executing enzymatic assays and interpreting their results.
• Experience with basic bioinformatics tools for the analysis of protein sequences and structure (BLAST searches, multiple sequence alignment, Pymol).
• Experience leading projects and small teams with multiple stakeholders and scientists.

£50k-£60k + excellent company benefits inc. share plan, development budget and enhanced leave.